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The paramagnetic contrast agent Gadolinium-BOPTA (BOPTA) is commonly used for magnetic resonance imaging (MRI) of the brain. BOPTA is transported across the blood-brain-barrier by an interaction with the largest neutral amino acid transporter in the brain, L-neutral amino acid transporter 1 (LAT1). The aim of this study is to determine the effect of interaction of Gadolinium-DTPA with the membrane properties of the nervous tissue on the observed diffusion characteristics of BOPTA in grey and white matter tissue in vitro. These characteristics include the self-diffusion coefficient (D), the effective diffusivity (De), the fractional volume of free space (Vf) and the effective volume fraction of free space (Vvf) and a qualitative comparison was made with the transverse relaxation time T2 dependence of the measured diffusion parameters. BOPTA inhibited the L-neutral amino acid transport by 86% in the cell experiments. The physiological uptake of gadolinium-BOPTA was lower than the uptake of gadolinium-DTPA. An indirect value for the ratio of the two tracers was estimated by an analysis of their diffusion characteristics. White matter tissue had higher fractional volumes of free space and thus lower tissue barriers than grey matter tissue. Gadolinium-DTPA diffusion in white matter tissue was less sensitive to local changes in tissue barriers than in the grey matter tissue. The diffusion of BOPTA in white matter tissue was more sensitive to tissue barriers than that in the grey matter tissue due
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