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Proteomic analysis of liver in the nutritional rat model of nonalcoholic fatty liver disease.
Proteomic methods have the potential to provide a complete snapshot of liver tissue in a noninvasive manner. This may have important implications for early diagnosis of liver diseases, as well as the development of novel therapeutic strategies. The aim of this study was to determine the differential protein expression in liver in the nutritional rat model of nonalcoholic fatty liver disease (NAFLD). Sprague-Dawley rats were fed a choline-deficient L-amino acid-defined diet (CDAA) or normal rat chow for 4 weeks. A total of 459 proteins were identified in three replicate 2-DE gels. The expression of 43 proteins was significantly changed in the livers of the CDAA group compared to the normal rat chow group. Overall, up-regulation of proteins and/or their activity was observed, including HSP60, apolipoprotein A-I, proteasome subunit β5, GST A1, P450, and 12-lipoxygenase, while down-regulation of proteins and/or their activity was observed, including the mitochondrial proteins dihydrolipoamide acetyltransferase and carnitine O-palmitoyltransferase-1, and metabolic proteins fructose-1,6-bisphosphatase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase. Importantly, the differentially expressed proteins can be considered as potential biomarkers and therapeutic targets for the management of NAFLD. This study provides a more comprehensive set of proteins that might be considered for the diagnosis and treatment of NAFLD.GOMA (Reuters) – The Congolese army on Thursday killed a rebel leader and wounded three others in a rebel camp in the Democratic Republic of Congo’s restive east, the army said.

It was not clear if the group, known
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