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the preparation of shmnp composites is achieved by adding the mnps to the polyamide 6 (pa6) matrix. however, as the most widely used commercially available pa6 has the chemical structure of nylon-6 (6), the synthesized shmnp composites must be fully characterized to confirm that they are indeed shmnp composites. the tem images of the prepared shmnp composites show that the addition of mnps to the pa6 matrix was possible. based on the particle size in the tem images, the average particle size of the mnps in the shmnp composites was calculated to be approximately 13 nm. the specific geometry of the shmnp composite samples enabled the calculation of the interaction ratio. the interaction ratio of the prepared shmnp composites was found to be lower than that of the uncoated mnps because of the lower dipole-dipole attraction between the mnps. using the calculation method, the dispersion state of the mnps in the prepared shmnp composites was improved, and the interaction radius between the mnps was reduced. this finding is supported by the tem images of the prepared shmnp composites. in these samples, the mnps formed smaller agglomerates than in uncoated mnps. in addition, the xrd results confirmed that the mnps in the shmnp composites had retained their magnetism. the magnetic properties of the prepared shmnp composites were measured by superconducting quantum interference device (squid) magnetometry and by vibrating sample magnetometer (vsm) measurements. the prepared shmnp composites showed an excellent magnetic response, with a saturation magnetization of 85 emu/g for the mnps and an induced magnetic moment of 0.0033 emu/g for the pa6 matrix. this value was equivalent to that of the strongest paramagnetic magnetite composite known in the literature. overall, it is clear that the magnetic properties of the prepared shmnp composites were maintained after the silica coating on the mnps.
Widespread use of biotechnology in the safety evaluation of novel food ingredients is still in its infancy. The purpose of this study was to identify alternative methodologies for the determination of enzymes by using cheap and readily available substrates in order to develop a preliminary screening method for the identification of novel enzyme inhibitors in extracts of food ingredients. The inhibitory activity of methanol crude extracts of 150 plant samples (n=150) towards three enzyme sources, porcine pancreatic lipase (PPL), human plasma cholinesterase (hPChE) and bovine serum albumin (BSA) was investigated in the presence of the analytical standard, orlistat. Several linear response curves were evaluated with varying enzyme concentrations (from 0 to 100 μg mL-1) and varying amounts of crude plant extract (from 0 to 100 μg mL-1) for PPL. For hPChE, PPL and BSA were incubated in the presence of methanol extracts from three different plant species. For both PPL and hPChE, the inhibition activity of plant extracts was determined with varying concentrations and varying amounts of crude plant extract. The results indicate that porcine pancreatic lipase could be inhibited by the crude extracts of all 150 plant samples. Generally, the enzyme inhibition by crude extracts was significant for extracts with concentration above 20 μg mL-1. Concentration and amount of the crude plant extract significantly affected the inhibitory activity of crude extracts. The most active plant extract was determined to be the rhizome of Dioscorea officinalis. The inhibition effects were even comparable to orlistat at a relatively high concentration. As the trend of response lines indicated, crude extracts derived from other plant sources could also inhibit porcine pancreatic lipase. Our results of this preliminary study provided evidence that methanol crude extracts of D. officinalis could be used in the inhibition of PPL, and this extract might be a good candidate for developing novel food ingredients and cosmeceuticals due to its strong inhibitory activity on PPL. The inhibition effects were also determined on a human cholinergic enzyme; human plasma cholinesterase. 5ec8ef588b
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